Gene Autry (Gene Autry) heads a cattlemen's association and calls on the inexperienced Jim Agnew (Rand Brooks) to negotiate the sale of 500 head of cattle. Jim ends up losing the cattle in a crooked poker game, however, and Gene and his sidekick, Frog (Smiley Burnette), set out to find the cheating gamblers. It soon becomes clear that the leader of the gamblers is none other than Asa Lock (Addison Richards), the dastardly father of Gene's romantic interest, Stephanie (Fay McKenzie).